Part:BBa_K2680524

pLac/ara-1

Repressed by LacI and activated by arabinose/AraC. Intended for use in oscillator creation. Sequence originally from Lutz and Bujard 1997; later used for the creation of oscillator from Stricker et al 2008.^1,2 Note that the upstream LacO site is required for functional repression via LacI (tetrameter) [See Stricker et al 2008 supplement].

References

¹Lutz, R., & Bujard, H. (1997). Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Research, 25(6), 1203–1210.

²Stricker, J., Cookson, S., Bennett, M. R., Mather, W. H., Tsimring, L. S., & Hasty, J. (2008). A fast, robust and tunable synthetic gene oscillator. Nature, 456(7221), 516–519. https://doi.org/10.1038/nature07389

Part Contributions

Group: iGEM23_Stony-Brook

Authors: Bushra Islam, Michelle Yang

Contribution Summary
Confocal microscopy was used to visually confirm that there is some level of difference in GFP expression as time passes. Images were taken with the oscillation expressed as well- which had not been previously documented with this hybrid promoter. We hope that our methods as well as the experiments we conducted will help other iGEM teams replicate imaging results for GFP oscillations or for proving proof of concept, like we have. 

Time period: 3:32:58
Time period: 3:39:28

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 425
  Illegal XhoI site found at 392
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]